Human BNIP3L(BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like) ELISA Kit
| Code | Size | Price |
|---|
| ELK9461-48T | 48T | £298.00 |
Quantity:
| ELK9461-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
BNIP3a; BNIP3H; BNIP3L; NIP3 like protein X; NIP3L; NIX; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Infection immunity;
Sample type:
Tissue homogenates, cell lysates and other biological fluids.
Sensitivity:
0.094 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like(BNIP3L) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
O60238