Horse PRKAa1(Protein Kinase, AMP Activated Alpha 1) ELISA Kit
| Code | Size | Price |
|---|
| ELK9474-48T | 48T | £298.00 |
Quantity:
| ELK9474-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Equine (Horse)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
AMPK; AMPKa1; PKA; ACACA kinase; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Enzyme & Kinase;Metabolic pathway;
Sample type:
Tissue homogenates, cell lysates and other biological fluids
Sensitivity:
0.061 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Alpha 1(PRKAa1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Alpha 1(PRKAa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Alpha 1(PRKAa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, AMP Activated Alpha 1(PRKAa1) in the samples is then determined by comparing the OD of the samples to the standard curve.