Chicken GPX4(Glutathione Peroxidase 4) ELISA Kit
| Code | Size | Price |
|---|
| ELK9491-48T | 48T | £298.00 |
Quantity:
| ELK9491-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Chicken (Gallus)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
MCSP; PHGPx; SnGPx; SnPHGPx; Phospholipid Hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
31.25-2000 pg/mL
Research Area:
Enzyme & Kinase;
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
7.2 pg/mL
Standard:
2000 pg/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 4(GPX4). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione Peroxidase 4(GPX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 4(GPX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Peroxidase 4(GPX4) in the samples is then determined by comparing the OD of the samples to the standard curve.