Rat PYD(pyridinoline) ELISA Kit
| Code | Size | Price |
|---|
| ELK9504-48T | 48T | £298.00 |
Quantity:
| ELK9504-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Rat
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
F1; FI; Fbg; Coagulation Factor I
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
12.5-800 ng/mL
Research Area:
Biochemical research
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
5.5 ng/mL
Standard:
800 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to pyridinoline(PYD). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to pyridinoline(PYD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain pyridinoline(PYD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of pyridinoline(PYD) in the samples is then determined by comparing the OD of the samples to the standard curve.