Cattle CASP8(Caspase 8) ELISA Kit
| Code | Size | Price |
|---|
| ELK9510-48T | 48T | £298.00 |
Quantity:
| ELK9510-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Bovine (Cattle)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.32-20 ng/mL
Research Area:
Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Neuro science;
Sample type:
Tissue homogenates, cell lysates and other biological fluids
Sensitivity:
0.115 ng/mL
Standard:
20 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 8(Cattle CASP8). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Caspase 8(Cattle CASP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 8(Cattle CASP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 8(Cattle CASP8) in the samples is then determined by comparing the OD of the samples to the standard curve.