Goat HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit
| Code | Size | Price |
|---|
| ELK9511-48T | 48T | £359.00 |
Quantity:
| ELK9511-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Caprine (goat)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
DnaK; 70 Kilodalton Heat Shock Proteins;Heat shock 70 kDa protein 1; 2; heat shock 70kDa protein 1A; HSP70; HSP70 1; HSP70 1; HSP70 2; HSP70 1A; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSP70; Heat Shock Protein 70
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
3.13-200 ng/mL
Research Area:
Tumor immunity;Infection immunity;Developmental science;
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
1.29 ng/mL
Standard:
200 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HeatShock Protein 70(HSP-70/HSPA9). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HeatShock Protein 70(HSP-70/HSPA9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HeatShock Protein 70(HSP-70/HSPA9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HeatShock Protein 70(HSP-70/HSPA9) in the samples is then determined by comparing the OD of the samples to the standard curve.