Cattle ALD(Aldosterone) ELISA Kit

ELK Biotechnology
Product Code: ELK9522
Product Group: ELISA Kits
CodeSizePrice
ELK9522-48T48T£261.00
Quantity:
ELK9522-96T96T£335.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Bovine (Cattle)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
ALD
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
31.25-2000 pg/mL
Research Area:
Metabolic pathway;Endocrinology;Hormone metabolism;
Sample type:
serum, plasma and other biological fluids
Sensitivity:
9.41 pg/mL
Standard:
2000 pg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Aldosterone(Cattle ALD) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aldosterone(Cattle ALD). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldosterone(Cattle ALD) in the samples is then determined by comparing the OD of the samples to the standard curve.