Human MKRN3(makorin ring-finger protein 3) ELISA Kit
| Code | Size | Price |
|---|
| ELK9528-48T | 48T | £298.00 |
Quantity:
| ELK9528-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Probable E3 ubiquitin-protein ligase makorin-3; RING finger protein 63; Zinc finger protein 127; D15S9; RNF63; ZNF127
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
15.63-1000 pg/mL
Research Area:
Signal transduction
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
6.5 pg/mL
Standard:
1000 pg/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to makorin ring-finger protein 3(MKRN3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to makorin ring-finger protein 3(MKRN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain makorin ring-finger protein 3(MKRN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of makorin ring-finger protein 3(MKRN3) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q13064