Horse AMH(Anti-Mullerian Hormone) ELISA Kit

ELK Biotechnology
Product Code: ELK9556
Product Group: ELISA Kits
CodeSizePrice
ELK9556-48T48T£359.00
Quantity:
ELK9556-96T96T£477.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Equine (Horse)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
MIF; MIH; MIS; M?llerian Inhibiting Factor; M?llerian Inhibiting Hormone; M?llerian Inhibiting Substance
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
468.75-30000 pg/mL
Research Area:
Endocrinology;Reproductive science;Hormone metabolism;
Sample type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
133.8 pg/mL
Standard:
30000 pg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Mullerian Hormone(AMH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Mullerian Hormone(AMH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Mullerian Hormone(AMH) in the samples is then determined by comparing the OD of the samples to the standard curve.