Sheep VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit
Code | Size | Price |
---|
ELK9568-48T | 48T | £359.00 |
Quantity:
ELK9568-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Sheep
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CD106; INCAM-100; L1CAM
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
78.13-5000 pg/mL
Research Area:
CD & Adhesion molecule;Tumor immunity;Infection immunity;
Sample type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
30 pg/mL
Standard:
5000 pg/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1(VCAM1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1(VCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Cell Adhesion Molecule 1(VCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Cell Adhesion Molecule 1(VCAM1) in the samples is then determined by comparing the OD of the samples to the standard curve.