General 4-HNE(4-Hydroxynonenal) ELISA Kit
| Code | Size | Price |
|---|
| ELK9581-48T | 48T | £298.00 |
Quantity:
| ELK9581-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Species Independent
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
0.63-40 ng/mL
Research Area:
Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.38 ng/mL
Standard:
40 ng/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 4-Hydroxynonenal(4-HNE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-Hydroxynonenal(4-HNE). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-Hydroxynonenal(4-HNE) in the samples is then determined by comparing the OD of the samples to the standard curve.