General TGF-b1(TransformingGrowth Factor Beta 1) ELISA Kit
| Code | Size | Price |
|---|
| ELK9583-48T | 48T | £298.00 |
Quantity:
| ELK9583-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Species Independent
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Cartilage-inducing factor;CED;Differentiation inhibiting factor;DPD1;LAP;Latency-associated peptide;Prepro transforming growth factor beta 1;TGF beta 1;TGF beta;TGF beta 1 protein;TGF-beta 1 protein;TGF-beta-1;TGF-beta-5;TGF-beta1;TGFB;Tgfb-1;tgfb1;TGFB1;TGFbeta;TGFbeta1;Transforming Growth Factor b1;Transforming Growth Factor beta 1;Transforming growth factor beta 1a;transforming growth factor beta-1;transforming growth factor;beta 1;Transforming Growth Factor-?1
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Cytokine;Tumor immunity;Infection immunity;
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.10 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TransformingGrowth Factor Beta 1(TGF-b1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TransformingGrowth Factor Beta 1(TGF-b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TransformingGrowth Factor Beta 1(TGF-b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TransformingGrowth Factor Beta 1(TGF-b1) in the samples is then determined by comparing the OD of the samples to the standard curve.