General F-E3(Free Estriol) ELISA Kit
| Code | Size | Price |
|---|
| ELK9646-48T | 48T | £261.00 |
Quantity:
| ELK9646-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Species Independent
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
F-E3; Free Estriol
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
0.63-40 ng/mL
Research Area:
Endocrinology;Reproductive science;Hormone metabolism;
Sample type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.218 ng/mL
Standard:
40 ng/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Free Estriol(F-E3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Free Estriol(F-E3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Free Estriol(F-E3) in the samples is then determined by comparing the OD of the samples to the standard curve.