Sheep NKB(Neurokinin B) ELISA Kit
| Code | Size | Price |
|---|
| ELK9648-48T | 48T | £298.00 |
Quantity:
| ELK9648-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Sheep
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
NK-B; TAC3; NKNB; ZNEUROK1; Neuromedin K; Tachykinin 3; Neurokinin Beta
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
12.5-800 pg/mL
Research Area:
Neuro science;Hormone metabolism;
Sample type:
Serum, plasma and other biological fluids.
Sensitivity:
3.17 pg/mL
Standard:
800 pg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neurokinin B(NKB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurokinin B(NKB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurokinin B(NKB) in the samples is then determined by comparing the OD of the samples to the standard curve.