Human SAM68(Src associated in mitosis 68 kDa protein) ELISA Kit
| Code | Size | Price |
|---|
| ELK9665-48T | 48T | £298.00 |
Quantity:
| ELK9665-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
KHDRBS1; SAM68; KH domain-containing; RNA-binding, signal transduction-associated protein 1; GAP-associated tyrosine phosphoprotein p62; Src-associated in mitosis 68 kDa protein; Sam68; p21 Ras GTPase-activating protein-associated p62; p68
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Signal transduction; Epigenetics and Nuclear Signaling
Sample type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
0.053 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Src associated in mitosis 68 kDa protein(SAM68). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Src associated in mitosis 68 kDa protein(SAM68). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Src associated in mitosis 68 kDa protein(SAM68), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Src associated in mitosis 68 kDa protein(SAM68) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q07666