Pig CLDN3(Claudin 3) ELISA Kit

ELK Biotechnology
Product Code: ELK9669
Product Group: ELISA Kits
CodeSizePrice
ELK9669-48T48T£298.00
Quantity:
ELK9669-96T96T£390.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Porcine (pig)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
C7orf1; CPE-R2; CPETR2; HRVP1; RVP1; Clostridium Perfringens Enterotoxin Receptor 2; Ventral Prostate.1-Like Protein; CPE-Receptor 2
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.32-20 ng/mL
Research Area:
Signal transduction;
Sample type:
serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
0.112 ng/mL
Standard:
20 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 3(CLDN3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Claudin 3(CLDN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 3(CLDN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 3(CLDN3) in the samples is then determined by comparing the OD of the samples to the standard curve.