Cattle CKB(Creatine Kinase, Brain) ELISA Kit
| Code | Size | Price |
|---|
| ELK9672-48T | 48T | £298.00 |
Quantity:
| ELK9672-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Bovine (Cattle)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CK-BB; CKBB; CK-B; CK-1
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
1.57-100 ng/mL
Research Area:
Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Neuro science;
Sample type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.59 ng/mL
Standard:
100 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Brain(Cattle CKB). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Creatine Kinase, Brain(Cattle CKB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase, Brain(Cattle CKB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Creatine Kinase, Brain(Cattle CKB) in the samples is then determined by comparing the OD of the samples to the standard curve.