Rat Trp(Tryptophan) ELISA Kit
| Code | Size | Price |
|---|
| ELK9740-48T | 48T | £261.00 |
Quantity:
| ELK9740-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Rat
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Trp
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
1.57-100 µg/mL
Research Area:
Enzyme & Kinase;
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.62 µg/mL
Standard:
100 µg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tryptophan(Trp) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tryptophan(Trp). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tryptophan(Trp) in the samples is then determined by comparing the OD of the samples to the standard curve.