Human CH50(50% complement hemolysis)ELISA Kit
| Code | Size | Price |
|---|
| ELK9747-48T | 48T | £298.00 |
Quantity:
| ELK9747-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CH50
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
2.35-150 U/mL
Research Area:
Immunology?Metabolism?Signal Transduction
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
1.19 U/mL
Standard:
150 U/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 50% complement hemolysis(CH50). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 50% complement hemolysis(CH50). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 50% complement hemolysis(CH50), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 50% complement hemolysis(CH50) in the samples is then determined by comparing the OD of the samples to the standard curve.