Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit
| Code | Size | Price |
|---|
| ELK9768-48T | 48T | £298.00 |
Quantity:
| ELK9768-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Canine (dog)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
15.63-1000 µg/mL
Research Area:
Diagnosis of diabetes mellitus
Sample type:
plasma and erythrocyte lysates
Sensitivity:
4.25 µg/mL
Standard:
1000 µg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glycated Hemoglobin A1c(HbA1c) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycated Hemoglobin A1c(HbA1c). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycated Hemoglobin A1c(HbA1c) in the samples is then determined by comparing the OD of the samples to the standard curve.