CAF(C-terminal fragement of agrin) ELISA Kit
| Code | Size | Price |
|---|
| ELK9769-48T | 48T | £298.00 |
Quantity:
| ELK9769-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CAF; c-terminal fragement of agrin
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
31.25-2000 pg/mL
Research Area:
Neuro science;Developmental science;Urology;
Sample type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
13.1 pg/mL
Standard:
2000 pg/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to c-terminal fragement of agrin(CAF). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to c-terminal fragement of agrin(CAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain c-terminal fragement of agrin(CAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of c-terminal fragement of agrin(CAF) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q13111