Rabbit CPT1A(Carnitine Palmitoyltransferase 1A, Liver) ELISA Kit
| Code | Size | Price |
|---|
| ELK9796-48T | 48T | £298.00 |
Quantity:
| ELK9796-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Rabbit
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Enzyme & Kinase;
Sample type:
Tissue homogenates, cell lysates and other biological fluids
Sensitivity:
0.062 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Palmitoyltransferase 1A, Liver(CPT1A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Carnitine Palmitoyltransferase 1A, Liver(CPT1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carnitine Palmitoyltransferase 1A, Liver(CPT1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carnitine Palmitoyltransferase 1A, Liver(CPT1A) in the samples is then determined by comparing the OD of the samples to the standard curve.