General PGE1(Prostaglandin E1) ELISA Kit
| Code | Size | Price |
|---|
| ELK9815-48T | 48T | £261.00 |
Quantity:
| ELK9815-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Species Independent
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Prostaglandin E1; PGE1
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
31.25-2000 pg/mL
Research Area:
Cell culture supernatant; Plasma; Saliva; Serum; Tissue Culture Media; Urine
Sample type:
Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine
Sensitivity:
9.55 pg/mL
Standard:
2000 pg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prostaglandin E1(PGE1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostaglandin E1(PGE1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin E1(PGE1) in the samples is then determined by comparing the OD of the samples to the standard curve.