Human P4HTM(Transmembrane prolyl 4-hydroxylase) ELISA Kit

EGLN4; FLJ20262; HIF prolyl hydroxylase PH4; HIF-PH4; HIF-prolyl hydroxylase 4; HIFPH4; HPH-4; Hypoxia inducible factor prolyl 4 hydroxylase; Hypoxia inducible factor prolyl hydroxylase; Hypoxia-inducible factor prolyl hydroxylase 4; P4H TM; P4H with transmembrane domain; P4H-TM; P4htm; P4HTM_HUMAN; PH 4; PH4; PHD4; Proline 4 hydroxylase; Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum); Prolyl hydroxlase domain containing 4; Transmembrane prolyl 4-hydroxylase;

3.5h

Sandwich

15.63-1000 pg/mL

Cardiovascular; Epigenetics and Nuclear Signaling; Cancer;

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

5.7 pg/mL

1000 pg/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane prolyl 4-hydroxylase(P4HTM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transmembrane prolyl 4-hydroxylase(P4HTM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane prolyl 4-hydroxylase(P4HTM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transmembrane prolyl 4-hydroxylase(P4HTM) in the samples is then determined by comparing the OD of the samples to the standard curve.

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