Mouse Tb4(Thymosin Beta 4) ELISA Kit

ELK Biotechnology
Product Code: ELK9849
Product Group: ELISA Kits
CodeSizePrice
ELK9849-48T48T£298.00
Quantity:
ELK9849-96T96T£390.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
TMSB4X; FX; PTMB4; TB4X; TMSB4; Thymosin,Beta 4,X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
3.13-200 ng/mL
Research Area:
Metabolic pathway;Endocrinology;Bone metabolism;
Sample type:
Serum, plasma and other biological fluids
Sensitivity:
0.87 ng/mL
Standard:
200 ng/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thymosin Beta 4(Tb4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thymosin Beta 4(Tb4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymosin Beta 4(Tb4) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P20065