Pig MDA(Malondialdehyde) ELISA Kit
Code | Size | Price |
---|
ELK9850-48T | 48T | £261.00 |
Quantity:
ELK9850-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Porcine (pig)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
MDA
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
31.25-2000 ng/mL
Research Area:
Metabolic pathway;Hepatology;Hormone metabolism;
Sample type:
serum, plasma and other biological fluids
Sensitivity:
9.15 ng/mL
Standard:
2000 ng/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Malondialdehyde(MDA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Malondialdehyde(MDA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Malondialdehyde(MDA) in the samples is then determined by comparing the OD of the samples to the standard curve.