NT-3 Antibody
| Code | Size | Price |
|---|
| PSI-38-305-0.05mg | 0.05mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Neurotrophin-3, Neurotrophic factor, Nerve Growth Factor-2 (NGF-2), HGNF
Application Note:
Sandwich ELISA:To detect hNT-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human NT-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-3.
Western Blot
To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
NT-3 is a neurotrophic factor structurally related tobeta-NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous system, and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical. Recombinant Human NT-3 is a noncovalently linked homodimer of two 13.6 kDa polypeptide monomers (240 total amino acid residues).
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNT-3. Anti-Human NT-3 specific antibody was purified by affinity chromatography employing immobilized hNT-3 matrix.
NCBI Gene ID #:
4908
NCBI Official Name:
neurotrophin 3
NCBI Official Symbol:
NTF3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P20783
Protein GI Number:
P20783.1
Purification:
NT-3 specific antibody was purified by affinity chromatography employing immobilized hNT-3 matrix
Research Area:
Neuroscience,Stem Cell
Swissprot #:
P20783
User NOte:
Centrifuge vial prior to opening.