IL-1 beta Antibody
| Code | Size | Price |
|---|
| PSI-38-306-0.5mg | 0.5mg | £476.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 0.25-0.50 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human IL-1 beta when used with ProSci?s Biotinylated Antigen Affinity Purified Anti-Human IL-1beta as the detection antibody at a concentration of approximately 0.25-0.50 μg/ml.
Western Blot
To detect Human IL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-1beta is 2.0-4.0 ng/lane, under reducing conditions and 4.0-8.0 ng/lane, under non-reducing conditions.
Western Blot
To detect Human IL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-1beta is 2.0-4.0 ng/lane, under reducing conditions and 4.0-8.0 ng/lane, under non-reducing conditions.
Background:
IL-1 beta is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. Recombinant Human IL-1 beta is a 17.3 kDa protein containing 153 amino acid residues.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced in BALB/c mice immunized with highly pure Recombinant Human IL-1beta. Anti-Human IL-1 beta-specific antibody was purified from cell culture by Protein G affinity chromatography.
NCBI Gene ID #:
3553
NCBI Official Name:
interleukin 1 beta
NCBI Official Symbol:
IL1B
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01584
Protein GI Number:
P01584.2
Purification:
IL-1 beta-specific antibody was purified from cell culture by Protein G affinity chromatography
Research Area:
COVID-19,Immunology,Cancer,Obesity,Neuroscience,Cell Cycle,Stem Cell,Other
Swissprot #:
P01584
User NOte:
Centrifuge vial prior to opening.