IL-2 Antibody

ProSci
Product Code: PSI-38-307
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-307-0.5mg0.5mg£476.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Images

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In a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-2 when used with  ProSci's Biotinylated Antigen Affinity Purified Anti-Human IL-2 as the detection antibody at a concentration of approximately 1.0- 2.0 ug/ml.
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In a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-2 when used with  ProSci's Biotinylated Antigen Affinity Purified Anti-Human IL-2 as the detection antibody at a concentration of approximately 1.0- 2.0 ug/ml.

Further Information

Additional Names:
Interleukin-2, T-cell growth factor (TCGF), Aldesleukin
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-2 when used with ProSci?s Biotinylated Antigen Affinity Purified Anti-Human IL-2 as the detection antibody at a concentration of approximately 1.0- 2.0 μg/ml.
Western Blot
To detect Human IL-2 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Background:
IL-2 is a powerful immunoregulatory lymphokine produced by T cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant Human IL-2 is a 15.5 kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced in mice immunized with highly pure Recombinant Human IL-2. Anti-Human IL-2-specific antibody was purified from cell culture by Protein A affinity chromatography.
NCBI Gene ID #:
3558
NCBI Official Name:
interleukin 2
NCBI Official Symbol:
IL2
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P60568
Protein GI Number:
P60568.1
Purification:
IL-2-specific antibody was purified from cell culture by Protein A affinity chromatography
Research Area:
COVID-19,Infectious Disease,Cancer,Obesity,Neuroscience,Cell Cycle,Stem Cell,Other,Immunology
Swissprot #:
P60568
User NOte:
Centrifuge vial prior to opening.