IL-3 Antibody
| Code | Size | Price |
|---|
| PSI-38-308-0.5mg | 0.5mg | £476.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Interleukin-3, MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 8pg/ml of Recombinant Human IL-3 when used with ProSci?s Biotinylated Antigen Affinity Purified Anti-Human IL-3 as the detection antibody at a concentration of approximately 0.50- 1.0 μg/ml.
Western Blot
To detect Human IL-3 by Western Blot analysis, this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-3 is 2.0-4.0 ng/lane, under reducing and non-reducing conditions.
Western Blot
To detect Human IL-3 by Western Blot analysis, this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-3 is 2.0-4.0 ng/lane, under reducing and non-reducing conditions.
Background:
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine. Recombinant Human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced in mice immunized with highly pure Recombinant Human IL-3. Anti-Human IL-3-specific antibody was purified from tissue culture supernatant by Protein G affinity chromatography.
NCBI Gene ID #:
3562
NCBI Official Name:
interleukin 3
NCBI Official Symbol:
IL3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P08700
Protein GI Number:
P08700.2
Purification:
IL-3-specific antibody was purified from tissue culture supernatant by Protein G affinity chromatography
Research Area:
Infectious Disease,Cancer,Other,Immunology,Cell Cycle,Stem Cell
Swissprot #:
P08700
User NOte:
Centrifuge vial prior to opening.