IL-12 Antibody

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ProSci
Product Code: PSI-38-309
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-309-0.5mg0.5mg£476.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

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In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 1000pg/ml Recombinant Human IL-12 when used with ProSci's Biotinylated Antigen Affinity Purified Anti-Human IL-12 as the detection antibody at a concentration of at least 0.25-0.5 ug/ml.
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In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 1000pg/ml Recombinant Human IL-12 when used with ProSci's Biotinylated Antigen Affinity Purified Anti-Human IL-12 as the detection antibody at a concentration of at least 0.25-0.5 ug/ml.

Documents

Further Information

Additional Names:
Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF p35: Interleukin-12 subunit alpha, IL-12 subunit p35, IL-12A, Cytotoxic Lymphocyte Maturation Factor 35 kDa subunit (CLMF p35), NK cell Stimulating Factor Chain 1 p40: Interleukin-12 subunit beta, IL-12 subunit p40, IL-12B, CytotoxicLymphocyte Maturation Factor 40 kDa subunit (CLMF p40), NK cell Stimulating Factor Chain 2
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml Recombinant Human IL-12 when used with ProSci?s Biotinylated Antigen Affinity Purified Anti-Human IL-12 as the detection antibody at a concentration of at least 0.25-0.5 μg/ml.
Western Blot
To detect Human IL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-12 is 0.50-1.0 ng/lane, under non-reducing conditions.
Background:
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-gamma-producing Th1 cells. Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced in BALB/c x ICR F1 mice immunized with highly pure Recombinant Human IL-12. Anti-Human IL- 12-specific antibody was purified from ascites fluid by Protein A affinity chromatography.
NCBI Gene ID #:
p35: 3592 p40: 3593
NCBI Official Name:
interleukin 12A, interleukin 12B
NCBI Official Symbol:
IL12A , IL12B
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
p35: P29459 p40: P29460
Protein GI Number:
P29459.2, P29460.1
Purification:
IL- 12-specific antibody was purified from ascites fluid by Protein A affinity chromatography
Research Area:
COVID-19,Immunology,Cancer,Stem Cell,Other
Swissprot #:
p35: P29459 p40: P29460
User NOte:
Centrifuge vial prior to opening.