IL-12 Antibody
| Code | Size | Price |
|---|
| PSI-38-311-0.5mg | 0.5mg | £476.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Murine IL-12 when used with ProSci?s Biotinylated Antigen Affinity Purified anti-Murine IL-12 as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
Western Blot
To detect Murine IL-12 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-12 is 1.0-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.
Western Blot
To detect Murine IL-12 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-12 is 1.0-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.
Background:
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-gamma-producing Th1 cells. Recombinant Murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues).
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced in Sprague Dawley rats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified from cell culture by Protein G affinity chromatography.
NCBI Gene ID #:
p35: 16159 p40: 16160
NCBI Official Name:
interleukin 12a, interleukin 12b
NCBI Official Symbol:
Il12a , Il12b
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
p35: P43431 p40: P43432
Protein GI Number:
P43431.1, P43432.1
Purification:
IL-12-specific antibody was purified from cell culture by Protein G affinity chromatography
Research Area:
COVID-19,Immunology,Cancer,Stem Cell,Other
Swissprot #:
p35: P43431 p40: P43432
User NOte:
Centrifuge vial prior to opening.