IL-10 Antibody
| Code | Size | Price |
|---|
| PSI-38-312-0.5mg | 0.5mg | £476.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
B-TCGF, CSIF, TGIF
Application Note:
Sandwich ELISA: In a sandwich ELISA (assuming 100 μl/well), a concentration of 8.0-10.0 μg/ml of this antibody will detect at least 2000pg/ml of Recombinant Human IL-10 when used with ProSci?s Biotinylated Antigen Affinity Purified anti-Human IL-10 as the detection antibody at a concentration of approximately 0.05- 0.10 μg/ml.
Western Blot
To detect Human IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-10 is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
Western Blot
To detect Human IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-10 is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
Background:
IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-alpha. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells. Recombinant Human IL-10 is an 18.6 kDa protein of 161 amino acid residues.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced in BALB/c mice immunized with highly pure Recombinant Human IL-10. Anti-Human IL-10-specific antibody was purified from tissue culture supernatant by Protein A affinity chromatography.
NCBI Gene ID #:
3586
NCBI Official Name:
interleukin 10
NCBI Official Symbol:
IL10
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P22301
Protein GI Number:
P22301.1
Purification:
IL-10-specific antibody was purified from tissue culture supernatant by Protein A affinity chromatography
Research Area:
COVID-19,Infectious Disease,Cancer,Neuroscience,Stem Cell,Immunology,Other
Swissprot #:
P22301
User NOte:
Centrifuge vial prior to opening.