sIL-2 Receptor alpha Antibody
| Code | Size | Price |
|---|
| PSI-38-315-0.05mg | 0.05mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
soluble IL-2 receptor, TAC-antigen, CD25 antigen
Application Note:
Sandwich ELISA: To detect Human sIL-2 Receptor alpha by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human sIL-2 Receptor alpha as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human sIL-2 Receptor alpha.
Western Blot
To detect Human sIL-2 Receptor alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sIL-2 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect Human sIL-2 Receptor alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sIL-2 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2Ralpha, IL-2R beta, and IL-2R gamma. The IL-2R alpha is a type I transmembrane protein consisting of a 219 amino acid extracellular domain, a 19 amino acid transmembrane domain and a 13 amino acid intracellular domain, which is not involved in the transduction of IL-2 signals. Proteolytic processing of IL-2Ralpha releases the entire extracellular domain of IL-2Ralpha, thereby generating a 219 amino acid soluble protein called soluble IL-2R alpha (sIL-2R alpha). The homodimeric form binds IL-2 (KD=10mM) and facilitates IL-2 signaling. The secreted sIL-2Ralpha is expressed on leukemia cells, lymphoma cells, and newly activated T and B cells, as well as on approximately 10% of NK cells. Recombinant Human sIL-2 Receptor alpha is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2R alpha. As a result of glycosylation, Recombinant Human sIL-2 Receptor alpha migrates with an apparent molecular mass of approximately 40-50 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-2 Receptor alpha. Anti-Human sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography employing an immobilized Human sIL-2 Receptor alpha matrix.
NCBI Gene ID #:
3559
NCBI Official Name:
interleukin 2 receptor subunit alpha
NCBI Official Symbol:
IL2RA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01589
Protein GI Number:
P01589.1
Purification:
sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography employing an immobilized Human sIL-2 Receptor alpha matrix
Research Area:
Infectious Disease,Immunology,Neuroscience,Other
Swissprot #:
P01589
User NOte:
Centrifuge vial prior to opening.