Eotaxin (CCL11) Antibody (biotin)
| Code | Size | Price |
|---|
| PSI-38-321-0.025mg | 0.025mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CCL11
Application Note:
Sandwich ELISA: To detect hEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 ? 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human Eotaxin as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hEotaxin.
Western Blot
To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Eotaxin is a CC chemokine that signals through the CCR3 receptor. It is produced by IFN-gamma-stimulated endothelial cells and TNF-activated monocytes. Eotaxin selectively chemoattracts eosinophils and, along with Eotaxin-2 and Eotaxin-3, plays a key role in the regulation of eosinophil recruitment in the asthmatic lung and in allergic reactions. Recombinant Human Eotaxin is an 8.3 kDa protein containing 74 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEotaxin. Anti-Human Eotaxin specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
6356
NCBI Official Name:
C-C motif chemokine ligand 11
NCBI Official Symbol:
CCL11
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P51671
Protein GI Number:
P51671.1
Purification:
Eotaxin specific antibody was purified by affinity chromatography and then biotinylated
Research Area:
Chemokines & Cytokines,Immunology,Other
Swissprot #:
P51671
User NOte:
Centrifuge vial prior to opening.