TPO Antibody
| Code | Size | Price |
|---|
| PSI-38-324-0.05mg | 0.05mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF
Application Note:
Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human TPO as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.
Western Blot
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
TPO is a lineage-specific growth factor produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor, and acts as an important regulator of circulating platelets. Human and murine TPO exhibit cross-species reactivity. The human TPO gene encodes for a 353 amino acid glycoprotein, which contains a 21 amino acid signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTPO. Anti-Human TPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.
NCBI Gene ID #:
7066
NCBI Official Name:
thrombopoietin
NCBI Official Symbol:
THPO
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P40225
Protein GI Number:
P40225.1
Purification:
TPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix
Research Area:
Other,Cell Cycle,Stem Cell
Swissprot #:
P40225
User NOte:
Centrifuge vial prior to opening.