IL-3 Antibody
| Code | Size | Price |
|---|
| PSI-38-326-0.05mg | 0.05mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor
Application Note:
Sandwich ELISA: To detect mIL-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine IL-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-3.
Western Blot
To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine. Recombinant Murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-3. Anti-Murine IL-3-specific antibody was purified by affinity chromatography employing immobilized Murine IL-3 matrix.
NCBI Gene ID #:
16187
NCBI Official Name:
interleukin 3
NCBI Official Symbol:
Il3
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P01586
Protein GI Number:
P01586.1
Purification:
IL-3-specific antibody was purified by affinity chromatography employing immobilized Murine IL-3 matrix
Research Area:
Infectious Disease,Cancer,Other,Immunology,Cell Cycle,Stem Cell
Swissprot #:
P01586
User NOte:
Centrifuge vial prior to opening.