IL-4 Antibody
| Code | Size | Price |
|---|
| PSI-38-328-0.05mg | 0.05mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
BCGF, BCDF, B cell stimulating factor (BSF-1)
Application Note:
Sandwich ELISA: To detect Murine IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine IL-4 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-4.
Western Blot
To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant Murine IL-4 is a 13.5 kDa globular protein containing 121 amino acid residues.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-4. Anti-Murine IL-4-specific antibody was purified by affinity chromatography employing immobilized Murine IL-4 matrix.
NCBI Gene ID #:
16189
NCBI Official Name:
interleukin 4
NCBI Official Symbol:
Il4
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P07750
Protein GI Number:
P07750.1
Purification:
IL-4-specific antibody was purified by affinity chromatography employing immobilized Murine IL-4 matrix
Research Area:
COVID-19,Apoptosis,Cancer,Other,Cell Cycle,Stem Cell,Immunology
Swissprot #:
P07750
User NOte:
Centrifuge vial prior to opening.