beta-NGF Antibody

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ProSci
Product Code: PSI-38-335
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-335-0.05mg0.05mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

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To detect Human beta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Human beta-NGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta-NGF.
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To detect Human beta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Human beta-NGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta-NGF.

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Further Information

Application Note:
Sandwich ELISA: To detect Humanbeta-NGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Humanbeta-NGF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Humanbeta-NGF.
Western Blot
To detect Humanbeta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Humanbeta-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure recombinant Human beta-NGF. Anti-Human beta-NGF specific antibody was purified by affinity chromatography employing immobilized Human beta-NGF matrix.
Physical State:
Lyophilized
Purification:
beta-NGF specific antibody was purified by affinity chromatography employing immobilized Human beta-NGF matrix
Research Area:
Immunology,Obesity,Neuroscience,Stem Cell,Other
User NOte:
Centrifuge vial prior to opening.