IL-12 Antibody
| Code | Size | Price |
|---|
| PSI-38-339-0.05mg | 0.05mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Interleukin-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF
p35: Interleukin-12 subunit alpha, IL-12 subunit p35, IL-12A, Cytotoxic Lymphocyte Maturation Factor 35 kDa subunit (CLMF p35), NK cell Stimulating Factor Chain 1
p40: Interleukin-12 subunit beta, IL-12 subunit p40, IL-12B, CytotoxicLymphocyte Maturation Factor 40 kDa subunit (CLMF p40), NK cell Stimulating Factor Chain 2
Application Note:
Sandwich ELISA: To detect hIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human IL-12 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12.
Western Blot
To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-gamma-producing Th1 cells. Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL12. Anti-Human IL-12 specific antibody was purified by affinity chromatography employing immobilized hIL12 matrix.
NCBI Gene ID #:
p35: 3592 p40: 3593
NCBI Official Name:
interleukin 12A, interleukin 12B
NCBI Official Symbol:
IL12A , IL12B
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
p35: P29459 p40: P29460
Protein GI Number:
P29459.2, P29460.1
Purification:
IL-12 specific antibody was purified by affinity chromatography employing immobilized hIL12 matrix
Research Area:
COVID-19,Immunology,Cancer,Stem Cell,Other
Swissprot #:
p35: P29459 p40: P29460
User NOte:
Centrifuge vial prior to opening.