IL-7 Antibody (biotin)

ProSci
Product Code: PSI-38-351
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-351-0.025mg0.025mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

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To detect Rat IL-7 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction
with Polyclonal Anti-Rat IL-7 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Rat IL-7.

To detect Rat IL-7 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction
with Polyclonal Anti-Rat IL-7 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Rat IL-7.

Further Information

Additional Names:
Lymphopoietin 1(LP-1), pre-B cell factor
Application Note:
Sandwich ELISA: To detect Rat IL-7 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 ? 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Rat IL-7 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Rat IL-7.
Western Blot
To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat IL-7 is 1.5 ? 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-7 is a hematopoietic growth factor that primarily affects early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors, such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant Rat IL-7 is a 15.0 kDa protein containing 130 amino acid residues.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Rat IL-7. Anti-Rat IL-7 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
25647
NCBI Official Name:
interleukin 7
NCBI Official Symbol:
Il7
NCBI Organism:
Rattus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P56478
Protein GI Number:
P56478.1
Purification:
IL-7 specific antibody was purified by affinity chromatography and then biotinylated
Research Area:
Cancer,Immunology,Stem Cell
Swissprot #:
P56478
User NOte:
Centrifuge vial prior to opening.