TFF-2 Antibody (biotin)
| Code | Size | Price |
|---|
| PSI-38-353-0.025mg | 0.025mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Trefoil Factor 2 (TFF2), Spasmolytic polypeptide (SP), Spasmolysin
Application Note:
Sandwich ELISA: To detect Human TFF-2 by sandwich ELISA (using 100 μl/well) a concentration of 0.25 ? 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti- Human TFF-2 as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human TFF-2.
Western Blot
To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human TFF-2 is 1.5 ? 3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human TFF-2 is 1.5 ? 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464). Recombinant Human TFF-2 is a 12.0 kDa polypeptide of 107 amino acid residues, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human TFF-2. Anti-Human TFF-2 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
7032
NCBI Official Name:
trefoil factor 2
NCBI Official Symbol:
TFF2
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q03403
Protein GI Number:
Q03403.2
Purification:
TFF-2 specific antibody was purified by affinity chromatography and then biotinylated
Research Area:
Cancer,Stem Cell
Swissprot #:
Q03403
User NOte:
Centrifuge vial prior to opening.