Osteopontin Antibody
| Code | Size | Price |
|---|
| PSI-38-354-0.05mg | 0.05mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Bone sialoprotein 1, Secreted phosphoprotein 1, SPP-1, Nephropontin, Uropontin
Application Note:
Sandwich ELISA: To detect Human Osteopontin by sandwich ELISA (using 100μl/well antibody solution), a concentration of 0.5 ? 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human Osteopontin as a detection antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Human Osteopontin.
Western Blot
To detect Human Osteopontin by Western Blot analysis this antibody can be used at a concentration of 0.1 ? 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Osteopontin is 1.5 ? 3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect Human Osteopontin by Western Blot analysis this antibody can be used at a concentration of 0.1 ? 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Osteopontin is 1.5 ? 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Osteopontin is a secreted glycoprotein that functions as a ligand to alphavbeta3 integrin and possibly other receptors. It binds tightly to hydroxyapatite and can act as a structural component of the extracellular mineralized matrix. Osteopontin is initially secreted as a 298 amino acid protein, which is subject to multiple post-translational modifications including glycosylation, phosphorylation, and specific proteolytic cleavages into various smaller molecular weight fragments. Osteopontin is expressed in a wide range of cells and tissues including osteoblasts, various tumor cell lines, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta and odontoblasts. In addition to its involvement in mineralized matrix formation, Osteopontin can also function as a cytokine that stimulates the release of IFNgamma and IL-12, while inhibiting the production of IL-10. Recombinant Human Osteopontin is a 298 amino acid protein, which, due to glycosylation, migrates at an apparent molecular weight of 60.0-65.0 kDa by SDS-PAGE analysis under reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human Osteopontin. Anti-Human Osteopontin-specific antibody was purified by affinity chromatography employing an immobilized Human Osteopontin matrix.
NCBI Gene ID #:
6696
NCBI Official Name:
secreted phosphoprotein 1
NCBI Official Symbol:
SPP1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P10451
Protein GI Number:
P10451.1
Purification:
Osteopontin-specific antibody was purified by affinity chromatography employing an immobilized Human Osteopontin matrix
Research Area:
Other,Cancer
Swissprot #:
P10451
User NOte:
Centrifuge vial prior to opening.