SDF-1 alpha (CXCL12) Antibody
| Code | Size | Price |
|---|
| PSI-38-356-0.05mg | 0.05mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Stromal-Cell Derived Factor-1, CXCL12, PBSF
Application Note:
Sandwich ELISA: To detect Rat SDF-1 alpha by sandwich ELISA (using 100μl/well antibody solution), a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Rat SDF-1 alpha as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat SDF-1 alpha.
Western Blot
To detect Rat SDF-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat SDF-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect Rat SDF-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat SDF-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
SDF-1 alpha andbeta are stromal-derived, CXC chemokines that signal through the CXCR4 receptor. SDF-1alpha andbeta chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV-suppressive activity in cells expressing the CXCR4 receptor. Human and murine SDF-1 proteins act across species. SDF-1 alpha andbeta contain the four highly conserved cysteine residues present in CXC chemokines. The mature SDF-1 alpha protein is the result of alternative splicing of the SDF-1 gene and contains 68 amino acid residues. Recombinant Rat SDF-1alpha is a 7.9 kDa protein containing 68 amino acid residues.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Rat SDF-1 alpha. Anti-Rat SDF-1 alpha-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1 alpha matrix.
NCBI Gene ID #:
24772
NCBI Official Name:
C-X-C motif chemokine ligand 12
NCBI Official Symbol:
Cxcl12
NCBI Organism:
Rattus norvegicus
Physical State:
Lyophilized
Protein Accession #:
Q9QZD1
Purification:
SDF-1 alpha-specific antibody was purified by affinity chromatography employing an immobilized Rat SDF-1 alpha matrix
Research Area:
Infectious Disease,Chemokines & Cytokines,Immunology,Neuroscience,Stem Cell,Other
Swissprot #:
Q9QZD1
User NOte:
Centrifuge vial prior to opening.