EPO Antibody
| Code | Size | Price |
|---|
| PSI-38-362-0.05mg | 0.05mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
Erythropoietin, Epoetin
Application Note:
Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Human EPO as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of Recombinant Human EPO.
Western Blot
To detect Human EPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect Human EPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Erythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. The differentiation of CFU-E (Colony Forming Unit-Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. ProSci?s Human EPO contains 166 amino acid residues and has a calculated molecular weight of approximately 18.4 kDa. As a result of glycosylation, Recombinant Human EPO migrates with an apparent molecular mass of 37.0 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography employing an immobilized Human EPO matrix.
NCBI Gene ID #:
2056
NCBI Official Name:
erythropoietin
NCBI Official Symbol:
EPO
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01588
Protein GI Number:
P01588.1
Purification:
EPO-specific antibody was purified by affinity chromatography employing an immobilized Human EPO matrix
Research Area:
Immunology,Neuroscience,Cell Cycle,Stem Cell
Swissprot #:
P01588
User NOte:
Centrifuge vial prior to opening.