BD-5 Antibody
| Code | Size | Price |
|---|
| PSI-38-370-0.05mg | 0.05mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Shipping:
Blue Ice
Storage:
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
BD5, DEFB5, DEFB105a, Beta-Defensin 5
Application Note:
Sandwich ELISA: To detect hBD-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human BD-5 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBD-5.
Western Blot
To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot
To detect hBD-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from thebeta-defensins by the pairing of their three disulfide bonds. To date, six humanbeta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant Human BD-5 is a 5.8 kDa protein containing 51 amino acid residues
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-5. Anti-Human BD-5 specific antibody was purified by affinity chromatography employing immobilized hBD-5 matrix.
NCBI Gene ID #:
245908
NCBI Official Name:
defensin beta 105A
NCBI Official Symbol:
DEFB105A
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q8NG35
Protein GI Number:
Q8NG35.1
Purification:
BD-5 specific antibody was purified by affinity chromatography employing immobilized hBD-5 matrix
Research Area:
Chemokines & Cytokines,Immunology
Swissprot #:
Q8NG35
User NOte:
Centrifuge vial prior to opening.