KRIBIOLISA™ Bevacuzimab (AVASTIN)?ELISA

0 - 100 ng/ml

Colorimetric, 450nm

Enzyme Immunoassay for the quantitative determination of Bevacizumab in serum, plasma and cell culture supernatant. Bevacizumab is a recombinant Human IgG;? monoclonal antibody specific for all human vascular endothelial growth factor A (VEGF-A) isoforms. Additionally, Bevacizumab binds to and neutralizes all human VEGF-A isoforms an bioactive proteolytic fragments, but not mouse or rat VEGF. Krishgen PK kits, developed using the innovator drug as calibrator, are used for the quantitation of serum protein drug levels, and can be used for biosimilar and generics research as well. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Bevacizumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Bevacizumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Bevacizumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Bevacizumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Serum, Plasma, and Cell Culture Supernatant

1.56ng/ml