KRIBIOLISA Glatiramer Acetate (COPAXONE) ELISA

0 ng/ml - 4000 ng/ml

Colorimetric, 450nm

Enzyme Immunoassay for the Quantitative Determination of Glatiramer Acetate in cell culture supernatant and pharmaceutical preparations Glatiramer Acetate (Copaxone) is a mixture of random-sized peptides that are composed of the four amino acids found in myelin basic protein, namely glutamic acid, lysine, alanine, and tyrosine. Myelin basic protein is the antigen in the myelin sheaths of the neurons that stimulates an autoimmune reaction in people with MS, so the peptide may work as a decoy for the attacking immune cells. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The Glatiramer Acetate ELISA is a competitive immunoassay for the determination of Glatiramer Acetate. A varying concentration of unlabeled standard or sample and constant concentration of GA:HRP conjugate will bind in sequence to the antibodies coated on the microplate. Upon washing, unbound GA:HRP Conjugate will be removed. Bound GA:HRP complex will produce a soluble blue colored product after the addition of TMB Substrate. The enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of bound Glatiramer Acetate present in the standards or samples.

Serum and Plasma

100 ng/ml