KRIBIOLISA™ PEG ELISA
Code | Size | Price |
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KBBA20 | 1 x 96 wells | £761.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Shipping:
2-8 ℃C
Documents
Further Information
Calibration Range:
39pg/ml - 2500pg/ml
Detection Method:
Colorimetric, 450nm
Product Description:
Enzyme Immunoassay for the quantitative determination of PEG in
serum, plasma and cell culture supernatant
PEG is generally considered biologically inert and safe. However, studies of clinical safety are generally based
on adults, not children. The FDA has been asked to investigate the possible effects of PEG in laxatives for
children. Also, a minority of people are allergic to it. Allergy to PEG is usually discovered after a person has
been diagnosed with an allergy to an increasing number of seemingly unrelated products, including processed
foods, cosmetics, drugs, and other substances that contain PEG or were manufactured with PEG. When PEG
is chemically attached to therapeutic molecules (such as protein drugs or nanoparticles), it can be antigenic
sometimes, stimulating an anti-PEG antibody response in some patients. This effect has only been shown for a
few of the many available PEGylated therapeutics, but it has significant effects on clinical outcomes of affected
patients. Other than these few instances where patients have anti-PEG immune responses, it is generally
considered to be a safe component of drug formulations.
About the kit:
- Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
- Recovery rates are between 85 - 115%
- Ready to use with a standard protocol with break-apart pre-coated wells
- Validated as per US FDA guidelines for Bioassays
- Optimized for matrix effects to ensure higher sensitivity.
- Shelf life: 1 year
The method employs the quantitative sandwich enzyme immunoassay technique. Anti- PEG antibody is
pre-coated onto microwells. In the first step, samples and standards are pipetted into microwells. If
PEG i s present in samples or standards, it will form complex with Anti- PEG antibody, which is pre-
coated onto microwells. In the second step, Anti- PEG Biotin conjugate is pipetted and incubated. In the
third step, HRP Conjugate is pipetted and incubated. Free HRP conjugate will be removed by a washing
step. In the f o u r t h s t e p , T M B substrate is added to microwells and color develops proportionally to
the amount of PEG present in samples or standards. Color development is then stopped by addition of
stop solution. Absorbance is measured at 450 nm.
Sample Type:
Serum, plasma and cell culture supernatant
Sensitivity:
39 pg/ml