KRIBIOLISA™ Protein A ELISA
| Code | Size | Price |
|---|
| KBBP11 | 1 x 96 wells | £389.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: RUO
Shipping:
2-8 ℃C
Further Information
Calibration Range:
0 pg/ml - 4000 pg/ml
Detection Method:
Colorimetric, 450nm
Product Description:
Immunoassay for the measurement Natural & Structurally conserved
Recombinant Protein A in samples containing Human immunoglobulin
A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals. These antibodies are
generally purified through protein A column chromatography. This method of purification has the potential with
contamination of protein A, which might get leached out of the column and co-eluted with purified antibody. This
may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody.
The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as
well as screening product prior to its use.
About the kit:
- Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity.
- Recovery rates are between 85 - 115%
- Ready to use with a standard protocol with break-apart pre-coated wells
- Validated as per US FDA guidelines for Bioassays
- Optimized for matrix effects to ensure higher sensitivity.
- Shelf life: 1 year
This assay is based on the principle of Sandwich ELISA. Samples containing Protein A are reacted with anti-
Protein A antibody coated on the microtitre plates. The wells are washed to remove any unbound reactants as per
the wash procedure. Anti-Protein A HRP antibody is added to the wells and incubated. . The wells are washed to
remove any unbound reactants as per the wash procedure. simultaneously in the microtiter wells already coated
with affinity purified capture anti-Protein A antibody. The substrate 3,3?,5,5? Tetramethyl Benzidine is then reacted.
The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the
concentration of Protein A present.
Sample Type:
Cell Culture Supernatant and other Biological Preparations
Sensitivity:
Please refer to the current IFU for the updated calibrator range and sensitivity.